Kottmann A H, Zevnik B, Welte M, Nielsen P J, Köhler G
Max-Planck-Institut für Immunbiologie, Freiburg, FRG.
Eur J Immunol. 1994 Apr;24(4):817-21. doi: 10.1002/eji.1830240407.
The joining of immunoglobulin gene segments during B cell development consists of a tightly regulated series of rearrangement steps. A variety of experiments have suggested that transcription is involved in activating the locus as substrate for the V(D)J recombinase. Here, we have characterized a region located immediately upstream of the most J-proximal D element (DQ52), which contains both promoter and enhancer activities preferentially active in precursors of B cells. Interestingly, this DQ52 regulatory element is inevitably deleted in fully rearranged H chain genes. We propose that it is involved in the early activation and rearrangement events at the IgH locus.
B细胞发育过程中免疫球蛋白基因片段的连接由一系列严格调控的重排步骤组成。各种实验表明，转录参与激活该基因座，使其成为V(D)J重组酶的底物。在这里，我们鉴定了一个位于最靠近J的D元件（DQ52）上游紧邻的区域，该区域含有优先在B细胞前体中活跃的启动子和增强子活性。有趣的是，这个DQ52调控元件在完全重排的重链基因中不可避免地被删除。我们提出，它参与了免疫球蛋白重链基因座的早期激活和重排事件。
