Kettleborough C A, Ansell K H, Allen R W, Rosell-Vives E, Güssow D H, Bendig M M
MRC Collaborative Centre, Mill Hill, London.
Eur J Immunol. 1994 Apr;24(4):952-8. doi: 10.1002/eji.1830240426.
Enhanced expression of epidermal growth factor receptor (EGFR) occurs on a variety of malignant tissues thus making anti-EGFR antibodies possible agents for the diagnosis and therapy of human tumors. Standard hybridoma technology has been used successfully to isolate anti-EGFR antibodies from immunized mice and rats. This report demonstrates that phage-antibody libraries are an alternative, and more versatile, method for isolating antibodies from immunized mice. Anti-EGFR antibodies were isolated from phage-antibody libraries constructed not only from the spleen of an immunized mouse but also from the draining lymph node of an immunized mouse and from in vitro immunized mouse cells. Two of the single-chain Fv isolated from the phage-antibody libraries were engineered to create partially humanized whole antibody molecules.
表皮生长因子受体（EGFR）在多种恶性组织中表达增强，因此抗EGFR抗体成为人类肿瘤诊断和治疗的潜在药物。标准杂交瘤技术已成功用于从免疫的小鼠和大鼠中分离抗EGFR抗体。本报告表明，噬菌体抗体文库是从免疫小鼠中分离抗体的另一种更通用的方法。抗EGFR抗体不仅从免疫小鼠的脾脏构建的噬菌体抗体文库中分离得到，还从免疫小鼠的引流淋巴结以及体外免疫的小鼠细胞中分离得到。从噬菌体抗体文库中分离出的两个单链Fv被改造以产生部分人源化的完整抗体分子。
