Micellar electrokinetic chromatography of hydroxyproline and other secondary amino acids in biological samples with laser-induced fluorescence detection.
作者信息
Chan K C, Janini G M, Muschik G M, Issaq H J
机构信息
PRI/DynCorp, NCI Frederick Cancer Research and Development Center, MD 21702.
出版信息
J Chromatogr. 1993 Dec 22;622(2):269-73. doi: 10.1016/0378-4347(93)80276-a.
PMID:8150876
Abstract
Micellar electrokinetic chromatography (MEKC) with laser-induced fluorescence (LIF) was used for the rapid and sensitive detection of hydroxyproline in serum and hydrolyzed urine that were pre-column derivatized with 9-fluorenylmethyl chloroformate (FMOC). The application of the combined o-phthalaldehyde (OPA)/FMOC derivatization in MEKC for the selective detection of secondary amino acids in biological samples is investigated.
摘要
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