Yoshida M, Yokoo H, Tanaka T, Emoto H, Tanaka M
Department of Pharmacology, Kurume University School of Medicine, Japan.
Brain Res. 1994 Feb 4;636(1):111-4. doi: 10.1016/0006-8993(94)90183-x.
Infusion of baclofen (10(-4) M, 1 h) into the ventral tegmental area (VTA), the cell body site of mesolimbic dopamine (DA) neuron system in conscious rats, caused a decrease in both axonal and somatodendritic DA release in this neuron system, when monitored by in vivo microdialysis using two probes simultaneously placed in both the NAC and the VTA. Levels of the metabolite of DA, 3,4-dihydroxyphenylacetic acid (DOPAC) in the VTA decreased significantly in a similar manner following infused baclofen into the VTA, however, a pronounced increase in DOPAC outflow was observed in dialysates from the NAC. This dissociated changes in DA metabolism observed in the NAC may possibly be derived from regulatory mechanisms via an autoreceptor located in the DA nerve terminals.
在清醒大鼠中，将巴氯芬（10⁻⁴ M，1小时）注入腹侧被盖区（VTA），即中脑边缘多巴胺（DA）神经元系统的细胞体部位。当使用两个探针同时置于伏隔核（NAC）和VTA进行体内微透析监测时，该神经元系统的轴突和树突体DA释放均减少。将巴氯芬注入VTA后，VTA中DA的代谢产物3,4-二羟基苯乙酸（DOPAC）水平以类似方式显著降低，然而，在来自NAC的透析液中观察到DOPAC流出量显著增加。在NAC中观察到的DA代谢的这种解离变化可能源于通过位于DA神经末梢的自身受体的调节机制。
