Sakai R R, He P F, Yang X D, Ma L Y, Guo Y F, Reilly J J, Moga C N, Fluharty S J
Department of Animal Biology, School of Medicine, University of Pennsylvania, Philadelphia.
J Neurochem. 1994 May;62(5):2053-6. doi: 10.1046/j.1471-4159.1994.62052053.x.
Antisense oligonucleotides were developed to study the expression and function of angiotensin type 1 (AT1) receptors in cultured cells and brain. In both liver epithelial WB and neuroblastoma N1E-115 cells AT1 antisense oligomers substantially decreased AT1 receptor density, whereas angiotensin type 2 (AT2) receptors remained unchanged. Similarly, repeated intracerebroventricular injections of AT1 antisense oligomers in rats decreased AT1 receptor density in hypothalamic-thalamic-septal tissue, and AT2 receptors were unaffected. Intracerebroventricular antisense oligomers also attenuated drinking elicited by intracerebroventricular angiotensin II but not the cholinomimetic carbachol. Collectively, these results demonstrate that antisense oligonucleotides attenuate angiotensin receptor expression and function in behaving animals.
反义寡核苷酸被开发用于研究血管紧张素1型（AT1）受体在培养细胞和大脑中的表达及功能。在肝上皮WB细胞和神经母细胞瘤N1E - 115细胞中，AT1反义寡聚体均显著降低了AT1受体密度，而血管紧张素2型（AT2）受体则保持不变。同样，在大鼠中反复脑室内注射AT1反义寡聚体可降低下丘脑 - 丘脑 - 隔区组织中的AT1受体密度，且AT2受体未受影响。脑室内反义寡聚体还减弱了脑室内注射血管紧张素II引起的饮水行为，但对拟胆碱药卡巴胆碱引起的饮水行为无影响。总体而言，这些结果表明反义寡核苷酸可减弱行为动物体内血管紧张素受体的表达和功能。
