Dubois-Stringfellow N, Kolpack-Martindale L, Bautch V L, Azizkhan R G
Department of Biology, University of North Carolina at Chapel Hill 27599.
Am J Pathol. 1994 Apr;144(4):796-806.
Inoculation of an established endothelial cell line from transgenic mouse hemangiomas (Py-4-1) into histocompatible mice induced vascular tumor formation at the site of infection with 100% frequency. Histological and hematological studies revealed that the mice developed hemangiomas with hematological changes similar to those found in the Kasabach-Merritt syndrome in humans, including hemolytic anemia and thrombocytopenic purpura. Modifications in the red blood cell count, hemoglobin concentration, hematocrit, and platelet count were directly correlated with the size of the hemangioma. Thus transgenic endothelial cell injection into histocompatible mice provides an in vivo model system to study the pathobiology of hemangiomas as well as the investigation of angiogenesis inhibitors.
将源自转基因小鼠血管瘤（Py-4-1）的已建立内皮细胞系接种到组织相容性小鼠中，在感染部位以100%的频率诱导血管肿瘤形成。组织学和血液学研究表明，这些小鼠患上了血管瘤，伴有与人类卡萨巴赫-梅里特综合征中发现的类似血液学变化，包括溶血性贫血和血小板减少性紫癜。红细胞计数、血红蛋白浓度、血细胞比容和血小板计数的改变与血管瘤的大小直接相关。因此，将转基因内皮细胞注射到组织相容性小鼠中提供了一个体内模型系统，用于研究血管瘤的病理生物学以及血管生成抑制剂。
