不同肿瘤来源的癌胚抗原（CEA）的物理化学和免疫化学特性。

Physicochemical and immunochemical properties of carcinoembryonic antigen (CEA) from different tumour sources.

作者信息

De Young N J, Ashman L K

出版信息

Aust J Exp Biol Med Sci. 1978 Jun;56(3):321-31. doi: 10.1038/icb.1978.34.

DOI:10.1038/icb.1978.34

Abstract

The physical, chemical and immunochemical properties of carcinoembryonic antigen (CEA) purified from hepatic metastases of eight tumours, originating in the colon (6), stomach (1) and lung (1), have been examined. Differences were observed in the overall molecular charge, and also in the carbohydrate composition of the different preparations (both total % carbohydrate, and mole % of the individual sugars). Negligible differences in amino acid composition were found. Gel filtration analysis of these CEA preparations and an additional four partially purified preparations (from pancreatic, hepatic, breast and oesophageal tumour tissues) revealed a single CEA-active peak of similar molecular weight (about 200,000-300,000 daltons) in all preparations. Radioimmunoassay data for the twelve CEA preparations indicated that all preparations contain the same antigenic determinants, as detected by our antiserum, but that there are differences in the expression of these determinants in different preparations.

摘要

对从8例肿瘤肝转移灶中纯化得到的癌胚抗原（CEA）的物理、化学和免疫化学性质进行了研究，这些肿瘤原发于结肠（6例）、胃（1例）和肺（1例）。观察到不同制剂的整体分子电荷以及碳水化合物组成（总碳水化合物百分比和各单糖的摩尔百分比）存在差异。氨基酸组成的差异可忽略不计。对这些CEA制剂以及另外4种部分纯化制剂（来自胰腺、肝脏、乳腺和食管肿瘤组织）进行的凝胶过滤分析显示，所有制剂中均有一个分子量相似（约200,000 - 300,000道尔顿）的CEA活性峰。12种CEA制剂的放射免疫分析数据表明，所有制剂都含有我们抗血清所检测到的相同抗原决定簇，但这些决定簇在不同制剂中的表达存在差异。