豚鼠、小鼠和大鼠脑片中磷酸化胶质纤维酸性蛋白的特性
Properties of phosphorylated glial fibrillary acidic protein in brain slices of guinea pig, mouse and rat.
作者信息
Gonçalves C A, Salbego C G, Rodnight R
机构信息
Departamento de Bioquímica, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil.
出版信息
Braz J Med Biol Res. 1994 Jan;27(1):81-9.
PMID:8173533
Abstract
- Brain micro-slices from guinea pig, mouse and rat were incubated in Krebs-Ringer Na-HEPES buffered medium containing [32P]-phosphate and characterized by immunoblotting with polyclonal antibody to glial fibrillary acidic protein (GFAP). 2. GFAP presented small differences in two-dimensional electrophoretic mobility. 3. The phosphorylation of GFAP was dependent on Ca2+ in the incubation medium in adult animals. 4. Both the immunocontent and level of phosphorylation of GFAP were higher in hippocampus than in cerebral cortex of all three species.
摘要
- 将豚鼠、小鼠和大鼠的脑薄片在含有[32P] - 磷酸盐的Krebs - Ringer Na - HEPES缓冲培养基中孵育，并用针对胶质纤维酸性蛋白（GFAP）的多克隆抗体进行免疫印迹表征。2. GFAP在二维电泳迁移率上存在微小差异。3. 在成年动物中，GFAP的磷酸化依赖于孵育培养基中的Ca2 +。4. 在所有三个物种中，海马体中GFAP的免疫含量和磷酸化水平均高于大脑皮层。
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