Liu Z, Ghai J, Ostrow R S, McGlennen R C, Faras A J
Institute of Human Genetics, University of Minnesota Medical School, Minneapolis 55455.
Virology. 1994 Jun;201(2):388-96. doi: 10.1006/viro.1994.1306.
The transforming potential of the E6 open reading frame (ORF) of the human papillomavirus type 16 was investigated with transformation assays in cotransfections with an activated ras gene. The E6 ORF driven by the heterologous CMV promoter could fully transform baby rat kidney cells (BRK) in cooperation with ras. The transformed cells grew in soft agar and induced tumors in athymic nude mice. The E6 ORF with mutations at the splicing donor site, which only encodes the full length E6 but not E6*s, could also fully transform the BRK cells at a similar efficiency as the wild-type E6 ORF, indicating that the full-length E6 was sufficient for the transformed phenotype.
通过与激活的ras基因共转染的转化试验，研究了人乳头瘤病毒16型E6开放阅读框（ORF）的转化潜力。由异源巨细胞病毒（CMV）启动子驱动的E6 ORF可与ras协同完全转化幼鼠肾细胞（BRK）。转化后的细胞在软琼脂中生长，并在无胸腺裸鼠中诱发肿瘤。在剪接供体位点发生突变的E6 ORF，其仅编码全长E6而不编码E6*s，也能以与野生型E6 ORF相似的效率完全转化BRK细胞，这表明全长E6足以产生转化表型。
