在PCR扩增过程中通过掺入磷酸化寡核苷酸进行诱变。 - Suppr

Suppr

超能文献

Mutagenesis by incorporation of a phosphorylated oligo during PCR amplification.

作者信息

Michael S F

机构信息

University of North Carolina at Chapel Hill.

出版信息

Biotechniques. 1994 Mar;16(3):410-2.

PMID:8185910

Abstract

摘要

相似文献

Mutagenesis by incorporation of a phosphorylated oligo during PCR amplification.

Biotechniques. 1994 Mar;16(3):410-2.

Saturation mutagenesis by mutagenic oligonucleotide-directed PCR amplification (Mod-PCR).

Methods Mol Biol. 1996;57:311-21. doi: 10.1385/0-89603-332-5:311.

Site-directed mutagenesis in vitro by megaprimer PCR.

Methods Mol Biol. 1996;57:203-15. doi: 10.1385/0-89603-332-5:203.

Multiple mutant cDNAs from one reaction mixture using asymmetric primers in PCR.

Biotechniques. 1995 Oct;19(4):556-9.

Using PCR to extend the limit of oligonucleotide synthesis.

Biotechniques. 1992 Jan;12(1):44, 46, 48.

Site-directed mutagenesis using a rapid PCR-based method.

Methods Mol Biol. 1996;57:239-48. doi: 10.1385/0-89603-332-5:239.

Using PCR for rapid site-specific mutagenesis in large plasmids.

Methods Mol Biol. 1996;57:217-27. doi: 10.1385/0-89603-332-5:217.

Flip-PCR for DNA sequence motif inversion.

Biotechniques. 1994 Mar;16(3):414-6.

Targeted amplification of mutant strands for efficient site-directed mutagenesis and mutant screening.

Methods Mol Biol. 2010;634:147-55. doi: 10.1007/978-1-60761-652-8_11.

The "looped oligo" method for generating reference molecules for quantitative PCR.

Biotechniques. 1994 Nov;17(5):864-6.

引用本文的文献

The Uropathogenic Specific Protein Gene from and is a Novel Member of the TyrR and H-NS Regulons.

Microorganisms. 2020 Feb 26;8(3):330. doi: 10.3390/microorganisms8030330.

A pore-forming toxin requires a specific residue for its activity in membranes with particular physicochemical properties.

J Biol Chem. 2015 Apr 24;290(17):10850-61. doi: 10.1074/jbc.M114.615211. Epub 2015 Mar 10.

Specific binding of the pathogenic prion isoform: development and characterization of a humanized single-chain variable antibody fragment.

PLoS One. 2011 Jan 20;6(1):e15783. doi: 10.1371/journal.pone.0015783.

Evolution of eukaryal tRNA-guanine transglycosylase: insight gained from the heterocyclic substrate recognition by the wild-type and mutant human and Escherichia coli tRNA-guanine transglycosylases.

Nucleic Acids Res. 2011 Apr;39(7):2834-44. doi: 10.1093/nar/gkq1188. Epub 2010 Dec 3.

Autocatalytic processing of procathepsin B is triggered by proenzyme activity.

FEBS J. 2009 Feb;276(3):660-8. doi: 10.1111/j.1742-4658.2008.06815.x.

Biosynthesis of Staphylococcus aureus autoinducing peptides by using the synechocystis DnaB mini-intein.

Appl Environ Microbiol. 2007 Oct;73(19):6036-44. doi: 10.1128/AEM.00912-07. Epub 2007 Aug 10.

Heterogeneity in expression of the Escherichia coli colicin K activity gene cka is controlled by the SOS system and stochastic factors.

Mol Genet Genomics. 2007 Apr;277(4):391-401. doi: 10.1007/s00438-006-0185-x. Epub 2007 Jan 11.

Independent regulation of MucD, an HtrA-like protease in Pseudomonas aeruginosa, and the role of its proteolytic motif in alginate gene regulation.

J Bacteriol. 2006 Apr;188(8):3134-7. doi: 10.1128/JB.188.8.3134-3137.2006.

Phosphorylation by protein kinase CKII modulates the DNA-binding activity of a chloroplast nucleoid-associated protein.

Planta. 2004 Jun;219(2):298-302. doi: 10.1007/s00425-004-1215-8. Epub 2004 Feb 17.

Residues C123 and D58 of the 2-methylisocitrate lyase (PrpB) enzyme of Salmonella enterica are essential for catalysis.

J Bacteriol. 2003 Aug;185(16):4837-43. doi: 10.1128/JB.185.16.4837-4843.2003.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验