Tabakov V Iu, Voeĭkova T A, Tokmakova I L, Bolotin A P, Vavilova E Iu, Lomovskaia n D
Genetika. 1994 Jan;30(1):57-61.
A system for introduction of plasmids into industrial producers of antibiotics chlortetracycline and bialaphos using intergeneric conjugation of Escherichia coli and Streptomyces was developed. Low level stability of inheritance of autonomously replicating DNA in recipient strains was shown. Site-specific integration of the conjugative-integrative vector pTO1 provided stable plasmid maintenance within the chromosomes of Streptomyces aureofaciens and S. hygroscopicus. Phenomenon of disturbance in differentiation and antibiotic production, resulting from pTO1 integration into S. hygroscopicus chromosome, was discovered.
开发了一种利用大肠杆菌和链霉菌的属间共轭将质粒导入抗生素金霉素和双丙氨膦的工业生产菌株的系统。结果表明，自主复制DNA在受体菌株中的遗传稳定性较低。共轭整合载体pTO1的位点特异性整合使质粒在金色链霉菌和吸水链霉菌的染色体中得以稳定维持。还发现了pTO1整合到吸水链霉菌染色体中导致分化和抗生素生产受到干扰的现象。
