在利用烷烃的固氮细菌中乙炔还原测定法的无效性。
Invalidity of the acetylene reduction assay in alkane-utilizing, nitrogen-fixing bacteria.
作者信息
De Bont J A, Mulder E G
出版信息
Appl Environ Microbiol. 1976 May;31(5):640-7. doi: 10.1128/aem.31.5.640-647.1976.
Abstract
The cause of the failure of the C2H2-C2H4 assay for nitrogen-fixing bacteria growing on lower alkanes was studied. Acetylene was a strong competitive inhibitor of methane oxidation for methane-utilizing bacteria, as well as for the oxidation of lower alkanes by other bacteria, so that energy and reducing power were no longer available for the reduction of acetylene by nitrogenase. Nitrogen-fixing bacteria grown on alkanes may reduce acetylene when intermediates of alkane-breakdown or other substrates oxidizable in the presence of acetylene are supplied. Ethylene co-oxidation is not responsible for the failure of the test, because acetylene also inhibits this co-oxidation along with methane oxidation.
摘要
研究了在低级烷烃上生长的固氮细菌的C2H2-C2H4测定失败的原因。乙炔是甲烷利用细菌甲烷氧化以及其他细菌对低级烷烃氧化的强竞争性抑制剂，因此固氮酶不再有能量和还原力用于乙炔还原。当提供烷烃分解中间体或在乙炔存在下可氧化的其他底物时，在烷烃上生长的固氮细菌可能会还原乙炔。乙烯共氧化不是试验失败的原因，因为乙炔在抑制甲烷氧化的同时也抑制了这种共氧化。
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