来自紫花苜蓿的一个冷诱导基因编码一种与发育调控蛋白相似的双模块蛋白。
A cold-induced gene from Medicago sativa encodes a bimodular protein similar to developmentally regulated proteins.
作者信息
Castonguay Y, Laberge S, Nadeau P, Vézina L P
机构信息
Station de Recherches, Agriculture Canada, Sainte-Foy, Québec.
出版信息
Plant Mol Biol. 1994 Mar;24(5):799-804. doi: 10.1007/BF00029861.
A new cold-regulated (COR) gene, msa CIC, was isolated by differential screening of a cDNA library from cold-acclimated crowns of alfalfa (Medicago sativa L. cv. Apica). Transcripts of msa CIC were not detectable in unacclimated alfalfa and accumulated to higher levels in cold-acclimated plants of the cold-tolerant cv. Apica than in those of the cold-sensitive cv. CUF-101. The DNA sequence analysis of a full-length cDNA clone revealed that msa CIC encodes for a putative protein (MSACIC) of 166 amino acids with distinct proline-rich and hydrophobic domains. Protein sequence comparisons indicated that MSACIC is similar to a group of bimodular proteins that are developmentally regulated in other plant species.
通过对来自冷驯化苜蓿（Medicago sativa L. cv. Apica）冠部的cDNA文库进行差异筛选，分离出一个新的冷调节（COR）基因msa CIC。在未驯化的苜蓿中检测不到msa CIC的转录本，而在耐寒品种Apica的冷驯化植株中，其转录本积累水平高于冷敏感品种CUF - 101的冷驯化植株。对一个全长cDNA克隆进行的DNA序列分析表明，msa CIC编码一种推定的蛋白质（MSACIC），该蛋白质由166个氨基酸组成，具有明显的富含脯氨酸结构域和疏水结构域。蛋白质序列比较表明，MSACIC与其他植物物种中受发育调控的一组双模块蛋白质相似。
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