采用差示扫描量热法和热重分析法测定牛角膜中的水分含量。

Determination of the content of water in bovine corneas by differential scanning calorimetry and thermogravimetric analysis.

作者信息

DeMali K A, Williams T R

机构信息

Department of Chemistry, College of Wooster, Ohio 44691.

出版信息

Ophthalmic Res. 1994;26(2):105-9. doi: 10.1159/000267399.

DOI:10.1159/000267399

PMID:8196931

Abstract

The 'bound', 'bulk', and total water contents in bovine corneas were investigated using differential scanning calorimetry and thermogravimetric analysis. The amount of total water (expressed as a function of total wet mass) was observed to be 83.7 +/- 1.1% using lyophilization and 84.1 +/- 0.8% using thermogravimetric analysis. These values are not significantly different from one another, but some discrepancies are observed when these values are compared with the values reported in the literature, thus indicating a need for guidelines concerning sample handling and analysis of these tissues. The 'bound' water was observed to be 62.3 +/- 2.7 (% of total water) and the amount of 'bulk' water was calculated to be 20.8 +/- 2.5 (% of total water).

摘要

使用差示扫描量热法和热重分析法研究了牛角膜中的“结合”水、“大量”水和总含水量。通过冻干法测得的总水量（以总湿质量的函数表示）为83.7±1.1%，通过热重分析法测得的为84.1±0.8%。这些值彼此之间没有显著差异，但将这些值与文献报道的值进行比较时，发现了一些差异，这表明需要针对这些组织的样品处理和分析制定指导方针。观察到“结合”水为62.3±2.7（占总水量的百分比），计算得出“大量”水的量为20.8±2.5（占总水量的百分比）。