荧光假单胞菌P-26部分纯化蛋白酶的热稳定性

Thermal resistance of partially purified proteinase of Pseudomonas fluorescens P-26.

作者信息

Uplacksh V K, Mathur D K, Malik R K

机构信息

Dairy Microbiology Division, National Dairy Research Institute, Karnal, India.

出版信息

J Appl Bacteriol. 1994 Apr;76(4):356-60. doi: 10.1111/j.1365-2672.1994.tb01640.x.

DOI:10.1111/j.1365-2672.1994.tb01640.x

PMID:8200863

Abstract

Heat resistance of the Pseudomonas fluorescens P-26 proteinase in terms of D-value was studied in whole milk, skim milk, whey and 0.05 mol l-1 phosphate buffer at 72.5, 130, 135, 140, 145 and 150 degrees C subsequent to its partial purification through (NH4)2 SO4 precipitation (45-65% saturation) and solvent fractionation with 1.0 to 2.0 volumes of isopropanol. The D-value was maximum for the proteinase at all temperatures when determined in whole milk (D150 = 0.088).

摘要

在通过硫酸铵沉淀（45 - 65%饱和度）和用1.0至2.0体积异丙醇进行溶剂分级对荧光假单胞菌P - 26蛋白酶进行部分纯化之后，于72.5、130、135、140、145和150摄氏度下，在全脂牛奶、脱脂牛奶、乳清和0.05 mol l-1磷酸盐缓冲液中研究了该蛋白酶的耐热性（以D值表示）。在全脂牛奶中测定时，该蛋白酶在所有温度下的D值均为最大（D150 = 0.088）。