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编码NSF和SNAP分泌蛋白同源物的神经表达果蝇基因。

Neurally expressed Drosophila genes encoding homologs of the NSF and SNAP secretory proteins.

作者信息

Ordway R W, Pallanck L, Ganetzky B

机构信息

Laboratory of Genetics, University of Wisconsin-Madison 53706.

出版信息

Proc Natl Acad Sci U S A. 1994 Jun 7;91(12):5715-9. doi: 10.1073/pnas.91.12.5715.

DOI:10.1073/pnas.91.12.5715
PMID:8202553
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC44067/
Abstract

Several lines of investigation have now converged to indicate that the neurotransmitter release apparatus is formed by assembly of cytosolic proteins with proteins of the synaptic vesicle and presynaptic terminal membranes. We are undertaking a genetic approach in Drosophila melanogaster to investigate the functions of two types of cytosolic proteins thought to function in this complex: N-ethylmaleimide-sensitive fusion protein (NSF) and the soluble NSF attachment proteins (SNAPs). We have identified Drosophila homologs of the vertebrate and yeast NSF and SNAP genes. Both Drosophila genes encode polypeptides that closely resemble their vertebrate counterparts and are expressed in the nervous system; neither appears to be in a family of closely related Drosophila genes. These results indicate that the Drosophila NSF and SNAP genes are excellent candidates for mutational analysis of neurotransmitter release.

摘要

目前，多条研究线索都表明，神经递质释放装置是由胞质蛋白与突触小泡和突触前终末膜的蛋白组装而成。我们正在利用黑腹果蝇进行遗传学研究，以探究两种被认为在这一复合体中发挥作用的胞质蛋白的功能：N-乙基马来酰亚胺敏感融合蛋白（NSF）和可溶性NSF附着蛋白（SNAPs）。我们已经鉴定出了脊椎动物和酵母NSF及SNAP基因的果蝇同源物。这两个果蝇基因编码的多肽与它们的脊椎动物对应物非常相似，并且在神经系统中表达；它们似乎都不属于果蝇中紧密相关的基因家族。这些结果表明，果蝇NSF和SNAP基因是对神经递质释放进行突变分析的极佳候选基因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1086/44067/687802b55f22/pnas01134-0509-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1086/44067/3ba15de2691c/pnas01134-0507-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1086/44067/d2a6dd77742b/pnas01134-0508-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1086/44067/8bc41a6ae450/pnas01134-0508-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1086/44067/ef569e0f8bf8/pnas01134-0509-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1086/44067/687802b55f22/pnas01134-0509-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1086/44067/3ba15de2691c/pnas01134-0507-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1086/44067/d2a6dd77742b/pnas01134-0508-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1086/44067/8bc41a6ae450/pnas01134-0508-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1086/44067/ef569e0f8bf8/pnas01134-0509-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1086/44067/687802b55f22/pnas01134-0509-b.jpg

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