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[小鼠下颌下腺颗粒导管细胞性别二态性的超微结构形态计量学研究]

[Ultrastructural morphometric study of sex dimorphism in cells of the ductus granulosus of the submandibular glands of mice].

作者信息

De Assis G F, Sottovia Filho D, Stipp A C, Taga R

机构信息

Departamento de Morfologia, Faculdade de Odontologia da USP, Bauru, SP.

出版信息

Rev Bras Biol. 1994 Feb;54(1):21-9.

PMID:8209034
Abstract

The sexual dimorphism in the secretory cell of the granular convolated tubule of mouse submandibular gland was morphometrically studied in light and electronic microscope level. In semithin sections (0.5 micron) the nuclear volume (Bach, 1963) and the fractions of cellular volume occupied by the nucleus and by cytoplasm were determined (Chalkley, 1943). In twenty electronmicrographs per animal with a final magnification of x 15.000, using the Weibel's et al. (1966) multiproposition test system, were obtained relatives morphometrics data, which multiplied by the cytoplasmatic volume provided us the respective absolute values. The results showed a presence of a sexual dimorphism at subcellular level. The secretory cell of the male is higher and exhibit a greater volume when compared with that cells of the female. In the male that cells are characterized by a higher total volume of the endoplasmic reticulum, Golgi complex and secretory granules. The secretory granules and the condensation vacuoles size are more developed in male cells. The female secretory granular cells exhibit a greater absolute number of the condensation vacuoles and secretory granules, as well as, a higher mitochondria total volume and surface.

摘要

在光学显微镜和电子显微镜水平上,对小鼠下颌下腺颗粒曲管分泌细胞的性别二态性进行了形态计量学研究。在半薄切片(0.5微米)中,测定了核体积(巴赫,1963年)以及细胞核和细胞质所占细胞体积的比例(乔克利,1943年)。在每只动物的20张电子显微照片中,最终放大倍数为15000倍,使用韦贝尔等人(1966年)的多命题测试系统,获得了相对形态计量学数据,将其乘以细胞质体积,为我们提供了各自的绝对值。结果表明在亚细胞水平存在性别二态性。与雌性细胞相比,雄性分泌细胞更高且体积更大。在雄性中,这些细胞的特征是内质网、高尔基体复合体和分泌颗粒的总体积更高。雄性细胞中分泌颗粒和浓缩泡的尺寸更大。雌性分泌颗粒细胞表现出更多的浓缩泡和分泌颗粒绝对数量,以及更高的线粒体总体积和表面积。

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