咪达唑仑给药期间氟马西尼对猫软脑膜血管直径和局部脑血流量的影响。

Effects of flumazenil during administration of midazolam on pial vessel diameter and regional cerebral blood flow in cats.

作者信息

Kumano H, Shimomura T, Furuya H, Yomosa H, Okuda T, Sakaki T, Kuro M

机构信息

Department of Anaesthesiology, Nara Medical University, Japan.

出版信息

Acta Anaesthesiol Scand. 1993 Aug;37(6):567-70. doi: 10.1111/j.1399-6576.1993.tb03766.x.

DOI:10.1111/j.1399-6576.1993.tb03766.x

PMID:8213021

Abstract

We implanted closed cranial windows in ten cats in order to observe the response of pial vessel diameter by microscopy using fluorescein isothiocyanate-labeled dextran and regional cerebral blood flow (rCBF) by laser Doppler flowmetry during administration of midazolam and reversal with flumazenil. Midazolam was given at 0.8 mg.kg-1 x min-1 for 10 min and maintained at 0.04 mg.kg-1 x min-1 for 50 min (total 10 mg.kg-1). The diameter of arterioles and venules and rCBF showed no significant change. During the last 10 min of midazolam administration, flumazenil, given at 0.1 mg.kg-1 x min-1 for 10 min (total 1 mg.kg-1), caused an increase of MAP and rCBF (P < 0.01) at 5 min after infusion and diameter of larger arterioles (> 50 microns) and venules (50-100 microns) were dilated (P < 0.05). These results indicate that midazolam does not affect the diameter of cerebral arterioles and venules; however, the reversal effects of flumazenil against midazolam are transient vasodilation of larger arterioles accompanied by an elevation of MAP, and an increase in CBF.

摘要

我们在10只猫身上植入了闭合式颅骨视窗，以便在给予咪达唑仑并使用氟马西尼进行逆转的过程中，通过显微镜观察使用异硫氰酸荧光素标记的葡聚糖时软脑膜血管直径的反应，以及通过激光多普勒血流仪观察局部脑血流量（rCBF）。咪达唑仑以0.8mg·kg⁻¹·min⁻¹的剂量给药10分钟，然后以0.04mg·kg⁻¹·min⁻¹的剂量维持50分钟（总量为10mg·kg⁻¹）。小动脉和小静脉的直径以及rCBF均未显示出显著变化。在咪达唑仑给药的最后10分钟内，氟马西尼以0.1mg·kg⁻¹·min⁻¹的剂量给药10分钟（总量为1mg·kg⁻¹），在输注后5分钟时导致平均动脉压（MAP）和rCBF升高（P<0.01），并且较大的小动脉（>50微米）和小静脉（50 - 100微米）的直径扩张（P<0.05）。这些结果表明，咪达唑仑不影响脑小动脉和小静脉的直径；然而，氟马西尼对咪达唑仑的逆转作用是较大小动脉的短暂血管舒张，伴有MAP升高和CBF增加。