Wulff H, Lange J V
Bull World Health Organ. 1975;52(4-6):429-36.
The indirect immunofluorescent technique is a rapid method for identification of Lassa virus and Lassa virus antibody. In the study reported here, Lassa virus antigen was detected by this method in Vero cell cultures within 24 hours of their inoculation with an infected human blood specimen. A diagnosis could be made from field-collected specimens within 3 days of their receipt.Fluorescent antibodies against Lassa virus were detected in human serum as early as 7 to 10 days after onset of illness, and were detected as long as 61 months after infection. Complement fixing antibodies were not as long lasting.No antigenic differences were noted by the indirect immunofluorescence technique between several Lassa virus strains isolated from Nigeria, Liberia, and Sierra Leone over a 6-year period.
间接免疫荧光技术是一种快速鉴定拉沙病毒和拉沙病毒抗体的方法。在本文报道的研究中，用这种方法在接种了感染人类血液标本的Vero细胞培养物中，于接种后24小时内检测到了拉沙病毒抗原。收到现场采集的标本后3天内即可做出诊断。在发病后7至10天即可在人血清中检测到抗拉沙病毒的荧光抗体，感染后长达61个月仍可检测到。补体结合抗体持续时间不长。通过间接免疫荧光技术未发现从尼日利亚、利比里亚和塞拉利昂分离出的几种拉沙病毒株在6年期间存在抗原差异。
