Li Z, Alavi M Z, Wasty F, Galis Z, Moore S
Department of Pathology, McGill University, Montreal, Que., Canada.
Pathobiology. 1993;61(2):89-94. doi: 10.1159/000163766.
Proteoglycans (PGs), the essential component of the extracellular matrix, are implicated in the pathogenesis of atherosclerosis. In an experimental model of injury, PGs accumulate in the neointimal tissue parallel with lipid deposition. However, it is still not clear whether the PG accumulation is from active smooth muscle cell (SMC) production or is a consequence of trapping within neointima covered by endothelium. To study the effect of endothelial injury on PG synthesis, SMCs were cultured from normal aorta (N-SMC), neointima covered by regenerated endothelium (W-SMC) and neointima without endothelium (B-SMC). Using [35S]-Na2SO4, as a precursor in an in vitro incubation, the kinetics of PG synthesis were determined. PG synthesis by all three cell types increases as a function of time. It is significantly higher in the SMCs cultured from endothelium-denuded aortic explants (W- and B-SMC) than N-SMC. This finding indicates that endothelial injury stimulates PG synthesis by SMCs.
蛋白聚糖（PGs）是细胞外基质的重要组成部分，与动脉粥样硬化的发病机制有关。在损伤的实验模型中，PGs在新生内膜组织中与脂质沉积同时积累。然而，PG积累是源于平滑肌细胞（SMC）的活跃产生还是被内皮覆盖的新生内膜内截留的结果仍不清楚。为了研究内皮损伤对PG合成的影响，从正常主动脉（N-SMC）、再生内皮覆盖的新生内膜（W-SMC）和无内皮的新生内膜（B-SMC）中培养SMC。使用[35S]-Na2SO4作为体外孵育的前体，测定PG合成的动力学。所有三种细胞类型的PG合成均随时间增加。从内皮剥脱的主动脉外植体培养的SMC（W-SMC和B-SMC）中的PG合成显著高于N-SMC。这一发现表明内皮损伤刺激SMC合成PG。
