Okuno K, Shirayama Y, Ohnishi H, Yamamoto K, Ozaki M, Hirohata T, Nakajima I, Yasutomi M
First Department of Surgery, Kinki University School of Medicine, Osaka-Sayama, Japan.
Surg Today. 1993;23(9):795-9. doi: 10.1007/BF00311622.
The aim of this study was to establish a reproducible and quantitative liver metastasis model in mice. The in vitro colon 26 (C-26) cultured cell line was initially taken from an in vivo transplantable C-26 adenocarcinoma tumor mass using the standard enzymatic treatments, collagenase and DNAse. In vitro cultured cells x 10(4) were introduced into the portal vein of syngeneic BALB/c mice to induce liver metastases and, 3 weeks later metastatic foci were found in approximately 50% to 70% of the mice. In contrast, C-26 cells desialylated by neuraminidase (Nase) treatment greatly increased the incidence of hepatic metastases with countable hepatic colonies being found in all mice (100%). This result seems to be related to the liver-characteristic D-galactose receptors, since pre-injection with an excess amount of galactocerebroside completely prevented tumor colonization in the liver. Thus, although we cannot disregard the involvement of other adhesion molecules in this system as yet, our experimental model may become a useful tool for the analysis of hepatic metastases from colon cancer in the future.
本研究的目的是在小鼠中建立一种可重复的定量肝转移模型。体外培养的结肠26（C-26）细胞系最初是使用标准酶处理方法，即胶原酶和脱氧核糖核酸酶，从体内可移植的C-26腺癌肿瘤块中获取的。将体外培养的10⁴个细胞注入同基因BALB/c小鼠的门静脉以诱导肝转移，3周后在约50%至70%的小鼠中发现了转移灶。相比之下，经神经氨酸酶（Nase）处理去唾液酸化的C-26细胞大大增加了肝转移的发生率，所有小鼠（100%）均发现了可计数的肝菌落。这一结果似乎与肝脏特有的D-半乳糖受体有关，因为预先注射过量的半乳糖脑苷脂可完全阻止肿瘤在肝脏中定植。因此，尽管目前我们尚不能忽视该系统中其他黏附分子的参与，但我们的实验模型未来可能会成为分析结肠癌肝转移的有用工具。
