Wang F J, Reiss C
Institut Jacques Monod, C.N.R.S., Université Paris VII, France.
Biochem Mol Biol Int. 1993 Aug;30(5):969-81.
The collision of cotranscribing E. coli ternary complexes was brought about in vitro by forcing the first "leading" complex to stall before first template C, due to the absence of CTP, and then permitting initiation of a second "following" complex. Following collision, the transcript of the leading complex was increased in length by as much as 7 nt., despite the absence of CTP, but did not dissociate. Upon addition of CTP, the leading complex aborted at exactly the positions requiring incorporation of the next Cs', the following complex continued transcription. The observations point to the importance of linking appropriate promoter efficiency with transcriptional pausing times, and the role of transcriptional collisions in termination events.
通过使第一个“先导”复合物在第一个模板C之前停滞（由于缺乏CTP），然后允许第二个“后续”复合物起始，在体外实现了共转录的大肠杆菌三元复合物的碰撞。碰撞后，尽管缺乏CTP，先导复合物的转录本长度增加了多达7个核苷酸，但并未解离。加入CTP后，先导复合物恰好在需要掺入下一个C的位置处终止，后续复合物继续转录。这些观察结果表明将适当的启动子效率与转录暂停时间联系起来的重要性，以及转录碰撞在终止事件中的作用。
