Stauber W T, Fritz V K, Burkovskaya T E, Ilyina-Kakueva E I
Department of Physiology, West Virginia University, Morgantown 26506.
Exp Mol Pathol. 1993 Oct;59(2):87-94. doi: 10.1006/exmp.1993.1030.
The proteolytic enzyme, chymase, was used to identify mast cells in rat gastrocnemius muscles which were crush-injured or incised in order to determine if mast cells exhibited proliferation and degranulation. Some of the crush-injured rats were subjected to 0 g for 14 days after injury on the Cosmos 2044 satellite to study the effects of weightlessness on the mast cell response. A variety of ground-based injured models were used, including a group of hindlimb-unloaded animals acting as controls and testing the suitability of the hindlimb-unloaded animal as a model for muscle healing during weightlessness. In most cases, the numbers of mast cells and their apparent size increased after injury. When mast cell degranulation was evident, the granules containing chymase often were free in the loose connective tissue and along the edge of myofibers. The mast cell response was most exaggerated in animals subjected to 0 g and least visible in the hindlimb-unloaded ones. Thus, gravitational stress may influence mast cell physiology and the hindlimb-unloaded animal may not be a good model for investigating muscle healing.
蛋白水解酶糜酶被用于鉴定大鼠腓肠肌中的肥大细胞，这些腓肠肌受到了挤压损伤或切割，目的是确定肥大细胞是否表现出增殖和脱颗粒现象。一些挤压损伤的大鼠在受伤后于宇宙2044卫星上经历了14天的失重状态，以研究失重对肥大细胞反应的影响。使用了多种地面损伤模型，包括一组作为对照的后肢去负荷动物，并测试后肢去负荷动物作为失重期间肌肉愈合模型的适用性。在大多数情况下，损伤后肥大细胞的数量及其明显大小增加。当肥大细胞脱颗粒明显时，含有糜酶的颗粒常常游离于疏松结缔组织中以及肌纤维边缘。肥大细胞反应在失重动物中最为明显，而在后肢去负荷动物中最不明显。因此，重力应激可能影响肥大细胞生理学，而后肢去负荷动物可能不是研究肌肉愈合的良好模型。
