Stabholz A, Neev J, Liaw L H, Stabholz A, Khayat A, Torabinejad M
Department of Endodontics, Hebrew University-Hadassah, School of Dental Medicine, Jerusalem, Israel.
J Endod. 1993 Jun;19(6):267-71. doi: 10.1016/s0099-2399(06)80454-1.
Root hypersensitivity occurs as a result of exposed dentinal tubules. Various methods and materials have been tried in an attempt to occlude these tubules. The purpose of this investigation was to study by scanning electron microscope the effects of XeCl excimer laser on exposed dentinal tubules of human extracted teeth. Fifteen 3-mm-thick slices were cut at the cementoenamel junction from 15 extracted human teeth by an electric saw. By using a diamond bur to remove the cementum layer the dentinal tubules were exposed. Each slice was scored by a permanent marker into four equal quadrants. Three of the quadrants were lased for 4 s by XeCl excimer laser with fluences ranging from 0.5 to 7.0 J/cm2 and pulse repetition of 25 Hz. The unlased quadrant served as control. The specimens were mounted on a stub, sputter coated by gold, and examined by scanning electron microscope. Nonlased surfaces showed numerous exposed dentinal tubules. In contrast, all specimens lased at fluences of up to 1 J/cm2 showed the presence of melted dentin which closed the dentinal tubules. At fluences of 4 J/cm2 and higher, rupture of molten materials and exposure of dentinal tubules were noted. The results indicate the application of XeCl excimer laser at specific fluences can cause melting of dentin and closure of exposed dentinal tubules.
牙本质过敏症是由牙本质小管暴露引起的。人们尝试了各种方法和材料来封闭这些小管。本研究的目的是通过扫描电子显微镜研究XeCl准分子激光对人离体牙暴露牙本质小管的影响。用锯从15颗拔除的人牙的牙骨质牙釉质交界处切取15片3毫米厚的切片。用金刚砂车针去除牙骨质层,使牙本质小管暴露。每片切片用永久性记号笔划分为四个相等的象限。其中三个象限用XeCl准分子激光照射4秒,能量密度范围为0.5至7.0 J/cm²,脉冲重复频率为25 Hz。未照射的象限作为对照。将标本固定在短柱上,喷金镀膜,然后用扫描电子显微镜检查。未照射的表面显示有许多暴露的牙本质小管。相比之下,所有能量密度高达1 J/cm²照射的标本都显示有熔化的牙本质,封闭了牙本质小管。在能量密度为4 J/cm²及更高时,观察到熔融材料破裂和牙本质小管暴露。结果表明,在特定能量密度下应用XeCl准分子激光可导致牙本质熔化和暴露的牙本质小管封闭。