Jensen A L, Høier R, Pedersen H D
Small Animal Hospital and Central Laboratory, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark.
Zentralbl Veterinarmed A. 1993 Sep;40(7):539-45. doi: 10.1111/j.1439-0442.1993.tb00663.x.
The purpose of the present study was to evaluate a commercially available enzyme linked immunosorbent assay (ELISA) for the determination of the free thyroxine (FT4) concentration in canine plasma samples. The test kit Enzymun-Test (Boehringer Mannheim) for determination of FT4 concentrations in human serum and plasma was used in connection with the analyzer Enzymun-Test(Boehringer Mannheim) System ES-33 by following the assay protocol suggested by the manufacturer. To assist in the evaluation of the ELISA method, goals for the precision of the assay were established from the inter- and intraindividual coefficients of variation estimated from determination of the plasma FT4 concentration in blood samples collected weekly from 8 healthy dogs for a period of 4 weeks. The intra- and interassay coefficients of variation ranged from 6% to 13%, the proposed goals for the precision ranging between 10% and 15%. The detection limit of the assay was 2 pmol/l which is well below the FT4 concentration commonly observed in plasma from dogs, and the FT4 concentrations determined in plasma from healthy dogs were comparable to those reported in the literature. Hence, the ELISA method evaluated in this study offers a precise and accurate measurement of the FT4 concentration in canine plasma samples, the inter- and intraassay precision comparing favorably to the proposed specifications for assay precision using estimates of the inter- and intraindividual coefficients of variation.
本研究的目的是评估一种市售酶联免疫吸附测定（ELISA）法，用于测定犬血浆样本中的游离甲状腺素（FT4）浓度。采用用于测定人血清和血浆中FT4浓度的酶免疫检测试剂盒（宝灵曼公司），与酶免疫检测分析仪（宝灵曼公司）ES-33系统配套使用，并遵循制造商建议的检测方案。为了辅助评估ELISA方法，通过对8只健康犬连续4周每周采集的血液样本测定血浆FT4浓度所估算的个体内和个体间变异系数，确定了该检测方法的精密度目标。批内和批间变异系数在6%至13%之间，建议的精密度目标在10%至15%之间。该检测方法的检测限为2 pmol/l，远低于犬血浆中常见的FT4浓度，且健康犬血浆中测定的FT4浓度与文献报道的浓度相当。因此，本研究中评估的ELISA方法能够精确、准确地测量犬血浆样本中的FT4浓度，批内和批间精密度与使用个体内和个体间变异系数估算值得出的检测方法精密度建议标准相比具有优势。
