免疫原性决定簇激活T淋巴细胞。

T-lymphocyte activation by immunogenic determinants.

作者信息

Goodman J W, Fong S, Lewis G K, Kamin R, Nitecki D E, Der Balian G

出版信息

Adv Exp Med Biol. 1978;98:143-64. doi: 10.1007/978-1-4615-8858-0_8.

Abstract

Synthetic antigens have been of great value in elucidating the relationships between antigen structure and lymphocyte activation. The compound RAT behaves as a monofunctional antigen in guinea pigs and mice, inducing T-lymphocyte responses without appreciable circulating antibody, although the ABA-specific B cell population is expanded by immunization with the monovalent molecule. On the other hand, bifunctional antigens composed of one RAT moiety serving as a carrier and a second chemical group, either identical to or different from RAT, serving as a hapten, induced antibody responses. In such responses, T cell specificity was always directed against the RAT component. Using symmetrical bifunctional antigens with rigid or flexible spacers between the two determinants, marked differences in structural requirements for cell triggering, assessed by antigen-induced lymphocyte proliferation, and for cell cooperation, determined by antibody formation, were found. Rigidly spaced bifunctional antigens serve admirably for cooperation but poorly for T cell activation, underscoring the advantage of two-point binding for the latter.

摘要

合成抗原在阐明抗原结构与淋巴细胞激活之间的关系方面具有重要价值。化合物RAT在豚鼠和小鼠中表现为单功能抗原，可诱导T淋巴细胞反应，且无明显的循环抗体，尽管用单价分子免疫可使ABA特异性B细胞群体扩增。另一方面，由一个作为载体的RAT部分和第二个与RAT相同或不同的化学基团作为半抗原组成的双功能抗原可诱导抗体反应。在这种反应中，T细胞特异性总是针对RAT成分。使用在两个决定簇之间带有刚性或柔性间隔物的对称双功能抗原，发现通过抗原诱导的淋巴细胞增殖评估的细胞触发结构要求以及通过抗体形成确定的细胞合作结构要求存在显著差异。刚性间隔的双功能抗原非常适合细胞合作，但在T细胞激活方面表现不佳，这突出了两点结合对后者的优势。