Flaherty K M, Zozulya S, Stryer L, McKay D B
Beckman Laboratories for Structural Biology, Department of Cell Biology, Stanford University School of Medicine, California 94305.
Cell. 1993 Nov 19;75(4):709-16. doi: 10.1016/0092-8674(93)90491-8.
Recoverin, a recently discovered member of the EF hand superfamily, serves as a calcium sensor in vision. We report here the crystal structure of recombinant unmyristoylated recoverin at 1.9 A resolution. The four EF hands of the protein are arranged in a compact array that contrasts with the dumbbell shape of calmodulin and troponin C. A calcium ion is bound to EF hand 3, while EF hand 2 can bind samarium but not calcium in this crystal form. The other two EF hands have novel structural features that prevent or impair calcium binding. A concave hydrophobic surface formed by EF hands 1 and 2 may participate in the read out of calcium signals by recoverin and its homologs.
恢复蛋白是EF手超家族最近发现的成员，在视觉中作为钙传感器。我们在此报告重组非肉豆蔻酰化恢复蛋白的晶体结构，分辨率为1.9埃。该蛋白的四个EF手以紧密排列的形式存在，这与钙调蛋白和肌钙蛋白C的哑铃形状形成对比。一个钙离子与EF手3结合，而在这种晶体形式下，EF手2可以结合钐但不能结合钙。另外两个EF手具有阻止或损害钙结合的新结构特征。由EF手1和2形成的凹面疏水表面可能参与恢复蛋白及其同源物对钙信号的读出。
