Jalkanen S, Salmi M
National Public Health Institute, Turku, Finland.
Behring Inst Mitt. 1993 Aug(92):36-43.
Leukocyte extravasation is critically dependent on proper interactions between leukocytes and vascular endothelial cells. Although several molecules mediating these interactions have been identified, all the homing phenomena observed in vivo cannot be explained on the basis of the known adhesion molecules. To discover novel molecules involved in leukocyte migration we have made monoclonal antibodies against human endothelial cells. With the help of one such monoclonal antibody a novel endothelial cell molecule called vascular adhesion protein-1 (VAP-1) was found. Uniqueness of VAP-1 is evident on the basis of its expression pattern, molecular mass, functional properties and N-terminal amino acid sequence. VAP-1 mediates lymphocyte binding to endothelium in peripheral lymph nodes, tonsil and inflamed synovium. Therefore, VAP-1 is relevant to understanding of the physiologic and pathologic lymphocyte migration in man, and may be valuable for dissecting the molecular events of tissue-selective lymphocyte homing.
白细胞渗出严重依赖于白细胞与血管内皮细胞之间的适当相互作用。尽管已经鉴定出几种介导这些相互作用的分子，但体内观察到的所有归巢现象都无法基于已知的黏附分子来解释。为了发现参与白细胞迁移的新分子，我们制备了针对人内皮细胞的单克隆抗体。借助其中一种单克隆抗体，发现了一种名为血管黏附蛋白-1（VAP-1）的新型内皮细胞分子。VAP-1的独特性基于其表达模式、分子量、功能特性和N端氨基酸序列是显而易见的。VAP-1介导淋巴细胞在外周淋巴结、扁桃体和炎症滑膜中与内皮的结合。因此，VAP-1与理解人类生理性和病理性淋巴细胞迁移相关，并且对于剖析组织选择性淋巴细胞归巢的分子事件可能具有重要价值。
