Boersma A, Degand P
C R Acad Hebd Seances Acad Sci D. 1976 Sep 13;283(4):397-9.
An alpha-L-fucosidase has been purified from Rat epididymis homogenate in two steps: 1, ammonium sulfate precipitation (64 p. 100 saturation) 2, "DEAE-Sephadex A 50" chromatography. The purified glycosidase was obtained with good yield and with purification degree consistent with oligosaccharide on glycopeptide structural studies.
已通过两步从大鼠附睾匀浆中纯化出一种α-L-岩藻糖苷酶:1. 硫酸铵沉淀(64%饱和度);2. “DEAE-葡聚糖A 50”柱色谱法。纯化后的糖苷酶收率良好,纯化程度与糖肽结构研究中的寡糖一致。