Nakagawa H
Third Department of Medicine, Kyoto Prefectural University of Medicine, Japan.
Jpn J Hum Genet. 1993 Sep;38(3):257-66. doi: 10.1007/BF01874136.
Fluorescence in situ hybridization (FISH) with a chromosome 7 specific alpha satellite DNA probe was used to detect monosomy 7 in interphase and metaphase cells obtained from patients with myelodysplastic syndrome (MDS) and acute nonlymphocytic leukemia (ANLL). Chromosome analysis revealed monosomy 7, either alone or as part of a complex chromosome abnormality, in all cell samples. FISH analyses of 12 marrow samples and a blood sample using a chromosome 7 specific alpha satellite DNA probe revealed a single fluorescence spot in 80.5-97.5% of interphase cells indicating monosomy 7. In contrast, 83.5-92.0% of the same cells had two copies of chromosome 17 as two fluorescent spots were detected using a chromosome 17 specific alpha satellite DNA probe used as a positive control. The proportion of interphase cells with monosomy 7 did not correlated with the percentage of metaphase cells with monosomy 7 detected by conventional karyotyping or with the percentage of blast cells in the bone marrow.
采用7号染色体特异性α卫星DNA探针进行荧光原位杂交（FISH），以检测骨髓增生异常综合征（MDS）和急性非淋巴细胞白血病（ANLL）患者的间期细胞和中期细胞中的7号染色体单体。染色体分析显示，所有细胞样本中均存在7号染色体单体，其可为单独存在或作为复杂染色体异常的一部分。使用7号染色体特异性α卫星DNA探针，对12份骨髓样本和1份血液样本进行FISH分析，结果显示80.5% - 97.5%的间期细胞中有一个荧光点，提示存在7号染色体单体。相比之下，作为阳性对照，使用17号染色体特异性α卫星DNA探针检测时，83.5% - 92.0%的相同细胞中有两个荧光点，表明存在两条17号染色体。间期细胞中7号染色体单体的比例，与通过传统核型分析检测到的中期细胞中7号染色体单体的百分比，或与骨髓中原始细胞的百分比均无相关性。
