Kaul P, Sidhu H, Sharma S K, Thind S K, Nath R
Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh.
Biochem Mol Biol Int. 1993 Sep;31(1):83-93.
The present study was carried out to investigate the relation between erythrocyte oxalate flux rate and the pathogenesis of calcium oxalate renal stone disease having hyperoxaluria as the main risk factor. Male albino rats were made hyperoxaluric by feeding them diets viz. vitamin B6 deficient, vitamin B6 deficient + 51.7% galactose or fructose (serving as a sole source of carbohydrate), along with their respective pair-fed controls for 4 weeks. After 28 days of feeding, oxalate excretion was in the order of vitamin B6 deficient + galactose > galactose control > vitamin B6 deficient + fructose > vitamin B6 deficient > fructose control/vitamin B6 control. Whereas, transmembrane oxalate flux rate was in the order of vitamin B6 deficient > vitamin B6 deficient + galactose > vitamin B6 deficient + fructose > vitamin B6 control > galactose control > fructose control. No significant correlation (r = 0.304) was found between the urinary oxalate excretion and erythrocyte oxalate flux rates of various groups of rats. The study indicates that increase in oxalate excretion does not concomitantly increase the transmembrane oxalate flux in red blood cells.
本研究旨在探讨以高草酸尿症为主要危险因素的草酸钙肾结石病发病机制与红细胞草酸通量率之间的关系。通过给雄性白化大鼠喂食不同饮食使其产生高草酸尿症，即维生素B6缺乏饮食、维生素B6缺乏 + 51.7%半乳糖或果糖（作为唯一碳水化合物来源）饮食，并分别设置相应的配对喂食对照组，持续4周。喂食28天后，草酸排泄量顺序为：维生素B6缺乏 + 半乳糖组 > 半乳糖对照组 > 维生素B6缺乏 + 果糖组 > 维生素B6缺乏组 > 果糖对照组/维生素B6对照组。然而，跨膜草酸通量率顺序为：维生素B6缺乏组 > 维生素B6缺乏 + 半乳糖组 > 维生素B6缺乏 + 果糖组 > 维生素B6对照组 > 半乳糖对照组 > 果糖对照组。各实验组大鼠的尿草酸排泄量与红细胞草酸通量率之间未发现显著相关性（r = 0.304）。该研究表明，草酸排泄量增加并不会同时增加红细胞的跨膜草酸通量。
