通过中和抑制酶免疫测定法检测两种脑心肌炎病毒中和单克隆抗体上的共同独特型表位。
Detection of a shared idiotope on two encephalomyocarditis virus neutralizing monoclonal antibodies by neutralization inhibition enzyme immunoassay.
作者信息
Kraaijeveld C A, Oosterlaken T A, Vlaspolder F, van Dijk P W, Harmsen M, Fernández I M, Benaissa-Trouw B J, Snippe H
机构信息
Eijkman-Winkler Laboratory for Medical Microbiology, Utrecht University, The Netherlands.
出版信息
J Virol Methods. 1993 Oct;44(2-3):319-28. doi: 10.1016/0166-0934(93)90066-z.
Idiotypic cross-reactivity between encephalomyocarditis virus (EMCV) neutralizing monoclonal antibodies UM 21.1 (IgG2b) and UM 21.3 (IgG2a) was detected by neutralization inhibition enzyme immunoassay using polyclonal and monoclonal anti-idiotypic antibodies. One strongly cross-reactive anti-idiotypic monoclonal antibody, designated 21.1A5 (IgG2b), might recognize a recurrent idiotope on EMCV neutralizing antibodies but it did not induce EMCV neutralizing anti-anti-idiotypic antibodies in homologous BALB/c mice.
利用多克隆和单克隆抗独特型抗体，通过中和抑制酶免疫测定法检测到脑心肌炎病毒（EMCV）中和单克隆抗体UM 21.1（IgG2b）和UM 21.3（IgG2a）之间的独特型交叉反应性。一种强烈交叉反应的抗独特型单克隆抗体，命名为21.1A5（IgG2b），可能识别EMCV中和抗体上的一个重复独特位，但在同源BALB/c小鼠中它并未诱导产生EMCV中和抗抗独特型抗体。
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