针对主要细胞蛋白的多克隆抗血清检测到的几种密螺旋体的共同抗原和特异性抗原。

Common and specific antigens of several treponemes detected by polyclonal antisera against major cellular proteins.

作者信息

Nakamura Y, Umemoto T, Nakatani Y, Namikawa I, Wadood A

机构信息

Department of Oral Microbiology, School of Dentistry, Asahi University, Gifu, Japan.

出版信息

Oral Microbiol Immunol. 1993 Oct;8(5):288-94. doi: 10.1111/j.1399-302x.1993.tb00576.x.

DOI:10.1111/j.1399-302x.1993.tb00576.x

PMID:8265202

Abstract

Thirteen polypeptide antigens with molecular weights ranging from 34 kDa to 83 kDa were selected and their antigenic behaviors and distribution were examined in 12 strains of microorganisms including Treponema, Borrelia, Leptospira and Leptonema. Immunoblot analysis demonstrated that 45 kDa and 83 kDa polypeptides of Treponema socranskii subsp. buccale ATCC 35534, 53 kDa antigen of Treponema denticola ATCC 33520 and 44 kDa polypeptide of the strain G7201 were strain-specific. The 34, 62, 66 and 84 kDa polypeptide antigens were detected in all 8 treponemal strains examined. T. denticola ATCC 33520 and ATCC 35404 possessed 38 kDa, 48 kDa, 52 kDa and 72 kDa common polypeptide antigens. All 12 strains possessed the 84 kDa polypeptide antigen. Immunoelectron microscopy demonstrated that the 34 kDa and 38 kDa polypeptide antigens were located on the axial flagella and that other polypeptide antigens were located on the outer envelopes or wall-membrane complexes.

摘要

选择了13种分子量在34 kDa至83 kDa之间的多肽抗原，并在包括梅毒螺旋体、疏螺旋体、钩端螺旋体和纤毛螺旋体在内的12种微生物菌株中检测了它们的抗原行为和分布。免疫印迹分析表明，口腔梅毒螺旋体亚种ATCC 35534的45 kDa和83 kDa多肽、具核梭杆菌ATCC 33520的53 kDa抗原以及菌株G7201的44 kDa多肽具有菌株特异性。在所有检测的8种梅毒螺旋体菌株中均检测到34、62、66和84 kDa的多肽抗原。具核梭杆菌ATCC 33520和ATCC 35404具有38 kDa、48 kDa、52 kDa和72 kDa的共同多肽抗原。所有12种菌株均具有84 kDa的多肽抗原。免疫电子显微镜显示，34 kDa和38 kDa的多肽抗原位于轴丝上，其他多肽抗原位于外膜或壁膜复合物上。