一种来自地钱（Marchantia polymorpha L.）的凝集素

A lectin from the liverwort Marchantia polymorpha L.

作者信息

Adam K P, Becker H

机构信息

Fachrichtung 12.3 der Universität des Saarlandes, Pharmakognosie und Analytische Phytochemie, D-66041 Saarbrücken, Germany.

出版信息

Experientia. 1993 Dec 15;49(12):1098-100. doi: 10.1007/BF01929921.

DOI:10.1007/BF01929921

PMID:8270033

Abstract

Bryophytes have been screened for lectins. From the liverwort Marchantia polymorpha (Marchiantiales) a lectin could be purified to homogeneity using a combination of ultrafiltration, size exclusion chromatography and ion exchange chromatography. SDS polyacrylamide gel electrophoresis, size exclusion chromatography and electrospray mass spectroscopy showed that the lectin is a monomeric protein with a M(r) of 16,134.64 +/- 2.93. Marchantia polymorpha lectin agglutinates erythrocytes of different mammalia and exhibits carbohydrate specificity against complex carbohydrate structures. This is the first report of a lectin isolated from liverworts.

摘要

人们已经对苔藓植物进行了凝集素筛选。从地钱（Marchantiales目）多形苔中，通过超滤、尺寸排阻色谱和离子交换色谱相结合的方法，可以将一种凝集素纯化至同质状态。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、尺寸排阻色谱和电喷雾质谱分析表明，该凝集素是一种单体蛋白，相对分子质量为16,134.64±2.93。多形苔凝集素能凝集不同哺乳动物的红细胞，并对复合碳水化合物结构表现出碳水化合物特异性。这是从地钱中分离出凝集素的首次报道。