Okuda H, Shioda S, Nakai Y, Nakayama H, Okamoto M, Nakashima T
Department of Pharmacology, Nara Medical University, Kashihara, Japan.
Neurosci Lett. 1993 Oct 29;161(2):183-6. doi: 10.1016/0304-3940(93)90289-w.
Whether or not corticotropin-releasing factor (CRF) containing synaptic vesicles are located in axon terminals with nicotinic acetylcholine receptor (nAChR) in the median eminence (ME) of the rat was examined by electron microscopic double-labeling immunocytochemistry combining the pre-embedding avidin-biotin-peroxidase complex (ABC) method for nAChR with the post-embedding immunogold staining method for CRF. nAChR-like immunoreactivity (nAChR-LI) was found in the cell membranes of the axon terminals in the ME. CRF-like immunoreactivity (CRF-LI) was found in dense granular vesicles (about 100 nm in diameter) in the axon terminals. Double-labeling method revealed that some of nAChR-LI axon terminals were found to contain CRF-LI dense granular vesicles. The results indicate that nicotine may act on nAChR in axon terminals to release CRF.
通过将用于烟碱型乙酰胆碱受体（nAChR）的包埋前抗生物素蛋白-生物素-过氧化物酶复合物（ABC）方法与用于促肾上腺皮质激素释放因子（CRF）的包埋后免疫金染色方法相结合的电子显微镜双标记免疫细胞化学技术，研究了大鼠正中隆起（ME）中含有CRF的突触小泡是否位于具有nAChR的轴突终末。在ME的轴突终末细胞膜中发现了nAChR样免疫反应性（nAChR-LI）。在轴突终末的致密颗粒小泡（直径约100 nm）中发现了CRF样免疫反应性（CRF-LI）。双标记法显示，一些nAChR-LI轴突终末含有CRF-LI致密颗粒小泡。结果表明，尼古丁可能作用于轴突终末的nAChR以释放CRF。
