Lütticken C, Wegenka U M, Yuan J, Buschmann J, Schindler C, Ziemiecki A, Harpur A G, Wilks A F, Yasukawa K, Taga T
Institute for Biochemistry, RWTH Aachen, Germany.
Science. 1994 Jan 7;263(5143):89-92. doi: 10.1126/science.8272872.
Interleukin-6 (IL-6), leukemia inhibitory factor, oncostatin M, interleukin-11, and ciliary neurotrophic factor bind to receptor complexes that share the signal transducer gp130. Upon binding, the ligands rapidly activate DNA binding of acute-phase response factor (APRF), a protein antigenically related to the p91 subunit of the interferon-stimulated gene factor-3 alpha (ISGF-3 alpha). These cytokines caused tyrosine phosphorylation of APRF and ISGF-3 alpha p91. Protein kinases of the Jak family were also rapidly tyrosine phosphorylated, and both APRF and Jak1 associated with gp130. These data indicate that Jak family protein kinases may participate in IL-6 signaling and that APRF may be activated in a complex with gp130.
白细胞介素-6（IL-6）、白血病抑制因子、制瘤素M、白细胞介素-11和睫状神经营养因子与共享信号转导子gp130的受体复合物结合。结合后，这些配体迅速激活急性期反应因子（APRF）的DNA结合，APRF是一种与干扰素刺激基因因子-3α（ISGF-3α）的p91亚基具有抗原相关性的蛋白质。这些细胞因子导致APRF和ISGF-3α p91的酪氨酸磷酸化。Jak家族的蛋白激酶也迅速发生酪氨酸磷酸化，并且APRF和Jak1都与gp130相关联。这些数据表明Jak家族蛋白激酶可能参与IL-6信号传导，并且APRF可能在与gp130形成的复合物中被激活。
