血小板活化因子在内皮素-1诱导的大鼠系膜细胞增殖中的作用。

A role for platelet-activating factor in endothelin-1-induced rat mesangial cell proliferation.

作者信息

Montero A, Rodriguez-Barbero A, López-Novoa J M

机构信息

Departamento de Fisiología y Farmacología, Facultad de Medicina, Universidad de Salamanca, Spain.

出版信息

Eur J Pharmacol. 1993 Oct 26;243(3):235-40. doi: 10.1016/0014-2999(93)90180-p.

DOI:10.1016/0014-2999(93)90180-p

PMID:8276075

Abstract

Endothelin-1 stimulated the release of bioassayable platelet-activating factor-like material and the incorporation of acetate to PAF in rat mesangial cells, in a dose-dependent manner, with an EC50 of about 10(-9) M. Endothelin-1 also stimulated dose dependently [3H]thymidine incorporation into DNA, protein synthesis and cell growth. The platelet-activating factor antagonists BN-52021 (10(-5) M), and alprazolam (10(-5) M), reduced significantly endothelin-1-stimulated thymidine incorporation into DNA, protein synthesis and cell growth. Platelet-activating factor also stimulated significantly thymidine incorporation into DNA, cell proliferation and protein synthesis. In conclusion, the present results suggest that endothelin-1 induces mesangial cell proliferation through a mechanism involving among others, synthesis and release of platelet-activating factor by these cells.

摘要

内皮素-1以剂量依赖方式刺激大鼠系膜细胞释放生物活性可检测的血小板活化因子样物质，并刺激乙酸盐掺入血小板活化因子，其半数有效浓度（EC50）约为10^(-9)M。内皮素-1还剂量依赖性地刺激[3H]胸腺嘧啶核苷掺入DNA、蛋白质合成和细胞生长。血小板活化因子拮抗剂BN-52021（10^(-5)M）和阿普唑仑（10^(-5)M）显著降低内皮素-1刺激的胸腺嘧啶核苷掺入DNA、蛋白质合成和细胞生长。血小板活化因子也显著刺激胸腺嘧啶核苷掺入DNA、细胞增殖和蛋白质合成。总之，目前的结果表明，内皮素-1通过一种机制诱导系膜细胞增殖，其中包括这些细胞合成和释放血小板活化因子。