Guttikar G, Patil S, Goel C, Rane R K, Ghatpande S K, Joshi M V, Mulherkar L, Modak S P
Department of Zoology, University of Poona, Pune, India.
Indian J Exp Biol. 1993 Oct;31(10):803-7.
Four hundred and forty four full primitive streak stage chick embryos were cultured in vitro and 261 were transplanted with 1, 3 or 5 Hensen's nodes in the area opaca. Irrespective of the number of grafts, neural induction was observed in 90% cases. The development of control and grafted embryos and the size of blastoderm area were monitored at the time of grafting and after 20 hr. We find that the induced neural tissue and differentiated tissue of graft-origin neither fuse with the host embryonic axis, nor retard its development.
444个处于原条完全形成期的鸡胚在体外培养,261个在暗区移植了1、3或5个亨氏结。无论移植的数量如何,90%的病例中都观察到了神经诱导。在移植时和20小时后监测对照胚胎和移植胚胎的发育情况以及胚盘面积大小。我们发现,诱导的神经组织和移植来源的分化组织既不与宿主胚胎轴融合,也不阻碍其发育。