Berger B W, Johnson R C, Kodner C, Coleman L
Department of Dermatology, State University of New York at Stony Brook.
J Am Acad Dermatol. 1994 Jan;30(1):48-51. doi: 10.1016/s0190-9622(94)70006-0.
We recently demonstrated that cultivation of Borrelia burgdorferi from skin biopsy specimens obtained from erythema migrans lesions was an efficacious procedure to confirm the diagnosis of Lyme disease.
Our purpose was to investigate the efficacy of our microbiologic technique on blood samples obtained from patients with Lyme disease and erythema migrans.
Whole blood samples were obtained from 52 patients with erythema migrans and early localized or early disseminated Lyme disease and placed into polystyrene tubes containing 6 ml of modified Barbour-Stoenner-Kelly medium, processed, and examined for B. burgdorferi by dark-field microscopy.
B. burgdorferi was cultured from the blood of two of our 52 patients (4%). Clinically, both of these patients were considered to have early localized Lyme disease.
The culture of B. burgdorferi from the blood of patients with early Lyme disease does not appear to be an efficacious procedure to confirm the diagnosis of Lyme disease. However, the demonstration of spirochetemia in patients with erythema migrans without any extracutaneous evidence of disseminated illness does have therapeutic significance.
我们最近证明，从游走性红斑病变的皮肤活检标本中培养伯氏疏螺旋体是确诊莱姆病的有效方法。
我们的目的是研究我们的微生物学技术对莱姆病和游走性红斑患者血液样本的有效性。
从52例游走性红斑以及早期局限性或早期播散性莱姆病患者中采集全血样本，放入含有6毫升改良巴伯-斯托纳-凯利培养基的聚苯乙烯管中，进行处理，并通过暗视野显微镜检查伯氏疏螺旋体。
在我们的52例患者中有2例（4%）血液中培养出伯氏疏螺旋体。临床上，这两名患者均被认为患有早期局限性莱姆病。
从早期莱姆病患者血液中培养伯氏疏螺旋体似乎不是确诊莱姆病的有效方法。然而，在没有任何皮肤外播散性疾病证据的游走性红斑患者中证实存在螺旋体血症确实具有治疗意义。
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