Tkacheno A G, Chudinov A A
Mikrobiologiia. 1993 Jul-Aug;62(4):654-62.
The interrelation between the Escherichia coli transport processes of K+ and putrescine+2 was investigated under the inhibition and limitation of the main energy consuming potassium transport systems (Trk, Kdp). It was shown that the potassium addition to the potassium deprived putrescine loaded cells triggers the putrescine exit at the exchange on the potassium. The stoichiometry of putrescine +2/2K+ translocation provide the electroneutrality of this process. The putrescine gradient turnover changes the potassium transport direction on the opposite one. The submitted experimental data are the evidence of putrescine +2/2K+ antiporter existence. The role of this antiporter in the E. coli pH-homeostasis is discussed.
在主要消耗能量的钾转运系统（Trk、Kdp）受到抑制和限制的情况下，研究了大肠杆菌中钾离子和腐胺二价阳离子（putrescine+2）转运过程之间的相互关系。结果表明，向缺钾且装载了腐胺的细胞中添加钾离子会引发腐胺以钾离子交换的方式排出。腐胺二价阳离子（putrescine+2）/2个钾离子转运的化学计量关系保证了这一过程的电中性。腐胺梯度的翻转会使钾离子的转运方向发生相反改变。所提交的实验数据证明了腐胺二价阳离子（putrescine+2）/2个钾离子反向转运体的存在。文中还讨论了这种反向转运体在大肠杆菌pH稳态中的作用。
