Usumoto R, Okada Y, Tsuji T
First Department of Internal Medicine, Okayama University Medical School, Japan.
Dig Dis Sci. 1994 Jan;39(1):169-76. doi: 10.1007/BF02090078.
The effect of a proton pump inhibitor, omeprazole, on the rat gastric mucosal expression of carbohydrate antigens was studied. Type 2 chain N-acetyllactosamine was detected specifically on the apicocanalicular cell membranes of parietal cells. Pretreatment of rats with omeprazole profoundly suppressed the antigen expression, which followed the inhibition of gastric acid secretion. When omeprazole was discontinued, the antigen was reexpressed, which preceded the restoration of acid secretion. The antigen-negative tissues became antigen-positive when they were desialylated. Gastric membrane vesicles from the normal and omeprazole-treated rats were antigen-positive and -negative, respectively. SDS-PAGE revealed that a glycoprotein with an apparent molecular weight of 64-78 kDa carried type 2 chain N-acetyllactosamine. In the omeprazole-treated rats, the same molecular weight glycoprotein was positively immunostained only after desialylation. We concluded that: (1) the expression of type 2 chain N-acetyllactosamine was closely correlated with gastric acid secretion, and (2) the inhibition of acid secretion was accompanied by the sialylation of the parietal cell membrane glycoprotein.
研究了质子泵抑制剂奥美拉唑对大鼠胃黏膜碳水化合物抗原表达的影响。在壁细胞的顶小管细胞膜上特异性检测到了2型链N-乙酰乳糖胺。用奥美拉唑预处理大鼠可显著抑制抗原表达，这伴随着胃酸分泌的抑制。当停用奥美拉唑时，抗原重新表达，这先于胃酸分泌的恢复。去唾液酸化后，抗原阴性组织变为抗原阳性。正常大鼠和经奥美拉唑处理的大鼠的胃膜囊泡分别为抗原阳性和阴性。SDS-PAGE显示，一种表观分子量为64-78 kDa的糖蛋白携带2型链N-乙酰乳糖胺。在经奥美拉唑处理的大鼠中，相同分子量的糖蛋白仅在去唾液酸化后呈阳性免疫染色。我们得出结论：（1）2型链N-乙酰乳糖胺的表达与胃酸分泌密切相关，（2）胃酸分泌的抑制伴随着壁细胞膜糖蛋白的唾液酸化。
