Monestier M, Losman M J, Novick K E, Aris J P
Garden State Cancer Center, Newark, NJ.
J Immunol. 1994 Jan 15;152(2):667-75.
In H-2S mice, the administration of mercuric chloride results in the development of antinucleolar autoantibodies. These mice represent a valuable model to study the role of environmental factors in the development of systemic autoimmunity. We have obtained seven antinucleolar mAb from mercury-injected A.SW mice and characterized their specificities and V genes. All mAb immunoprecipitate the U3 and U8 ribonucleoprotein particles (RNP) and some (but not all) react with fibrillarin, the only currently characterized protein component of mammalian nucleolar RNP. Several VH and V kappa genes are recurrently used by these antinucleolar RNP mAb and their H chain CDR3 segments contain several acidic residues that may be important for binding to the cationic proteins composing the nucleolar RNP. Our results support the concept that in H-2S mice administration of mercury induces a specific loss of tolerance to nucleolar RNP.
在H-2S小鼠中，注射氯化汞会导致抗核仁自身抗体的产生。这些小鼠是研究环境因素在系统性自身免疫发展中作用的宝贵模型。我们从注射汞的A.SW小鼠中获得了七种抗核仁单克隆抗体，并对它们的特异性和V基因进行了表征。所有单克隆抗体都能免疫沉淀U3和U8核糖核蛋白颗粒（RNP），并且一些（但不是全部）与纤维蛋白原发生反应，纤维蛋白原是目前唯一已表征的哺乳动物核仁RNP的蛋白质成分。这些抗核仁RNP单克隆抗体反复使用几种VH和Vκ基因，并且它们的重链互补决定区3（CDR3）片段含有几个酸性残基，这些残基可能对于与构成核仁RNP的阳离子蛋白结合很重要。我们的结果支持这样一种观念，即在H-2S小鼠中，汞的施用会导致对核仁RNP的特异性耐受性丧失。
