在胸膜肺炎放线杆菌10型中，与appA具有低同源性的基因座中存在类hlyIA序列。

hlyIA-like sequence present in a genetic locus with low homology to appA in Actinobacillus pleuropneumoniae serotype 10.

作者信息

Nagai S, Takahashi K, Mitsui T, Yagihashi T

机构信息

Nippon Institute for Biological Science, Tokyo, Japan.

出版信息

J Vet Med Sci. 1993 Oct;55(5):813-9. doi: 10.1292/jvms.55.813.

DOI:10.1292/jvms.55.813

PMID:8286536

Abstract

Using the appA gene encoding Actinobacillus pleuropneumoniae-RTX-toxin II (ApxII) as a probe, Southern hybridization demonstrated the presence of appA-sequence in all 12 serotypes except serotype 10. Serotype 10 strains had a genetic locus with low homology to appA (tentatively designated Lha). DNA sequence analysis revealed that Lha contained a part of hlyIA gene encoding a 105-kDa hemolysin ApxI. Southern hybridization demonstrated the presence of Lha (hlyIA) sequence not only in serotype 10 but also in serotypes 1, 5a, 5b, 9, and 11, which had strong hemolytic activity with sheep red blood cells and were highly virulent for mice. These results suggest that ApxI may be associated with the virulence phenotype of the organism.

摘要

以编码胸膜肺炎放线杆菌RTX毒素II（ApxII）的appA基因作为探针，Southern杂交显示除血清型10外，所有12种血清型中均存在appA序列。血清型10菌株有一个与appA同源性较低的基因座（暂命名为Lha）。DNA序列分析表明，Lha包含编码105 kDa溶血素ApxI的hlyIA基因的一部分。Southern杂交显示，Lha（hlyIA）序列不仅存在于血清型10中，也存在于血清型1、5a、5b、9和11中，这些血清型对绵羊红细胞具有很强的溶血活性，且对小鼠具有高度毒性。这些结果表明，ApxI可能与该生物体的毒力表型有关。