Zhu Y, Cantor C R, Smith C L
Division of Chemical Biodynamics, Lawrence Berkeley Laboratory, University of California, Berkeley 94720.
Genomics. 1993 Nov;18(2):199-205. doi: 10.1006/geno.1993.1455.
Portions of 16 chromosome 21 NotI linking clones were sequenced. These linking clone sequences represent sequence-tagged restriction sites that are potentially useful for finding genes and for finer genome mapping and sequencing. All of the clones were G+C rich (54 to 83%). CpG and GpC dinucleotide frequencies were very close to the expected values based on base composition and were very similar in 15 of the clones. Most of the NotI linking clones were derived from CpG islands, which are often associated with genes. Five NotI linking clones had a high potential for coding regions; 7 additional clones may also contain coding regions. The NotI linking clones had many short homologous regions, but no extensive homologies either with each other or with GenBank sequences.
对16个21号染色体NotI连接克隆的部分区域进行了测序。这些连接克隆序列代表了序列标签限制位点，它们对于寻找基因以及进行更精细的基因组图谱绘制和测序可能是有用的。所有克隆的G+C含量都很高（54%至83%）。CpG和GpC二核苷酸频率与基于碱基组成的预期值非常接近，并且在15个克隆中非常相似。大多数NotI连接克隆源自CpG岛，CpG岛通常与基因相关。5个NotI连接克隆具有编码区的高潜力；另外7个克隆也可能包含编码区。NotI连接克隆有许多短的同源区域，但彼此之间以及与GenBank序列都没有广泛的同源性。
