Shieh M T, Spear P G
Department of Microbiology-Immunology, Northwestern University Medical School, Chicago, Illinois 60611.
J Virol. 1994 Feb;68(2):1224-8. doi: 10.1128/JVI.68.2.1224-1228.1994.
The entry of enveloped viruses into animal cells and the cell-to-cell spread of infection via cell fusion require the membrane-fusing activity of viral glycoproteins. This activity can be dependent on variable cell factors or triggered by environmental factors. Here we show that cell fusion induced by herpes simplex virus glycoproteins is dependent on the presence of cell surface glycosaminoglycans, principally heparan sulfate, or on the addition of heparin to the medium. The role of the glycosaminoglycan is probably to alter the conformation of a viral heparin-binding glycoprotein required for the fusion.
包膜病毒进入动物细胞以及通过细胞融合实现感染的细胞间传播需要病毒糖蛋白的膜融合活性。这种活性可能依赖于可变的细胞因子或由环境因素触发。在这里，我们表明单纯疱疹病毒糖蛋白诱导的细胞融合依赖于细胞表面糖胺聚糖（主要是硫酸乙酰肝素）的存在，或者依赖于向培养基中添加肝素。糖胺聚糖的作用可能是改变融合所需的病毒肝素结合糖蛋白的构象。
