Friedman M G, Haikin H, Leventon-Kriss S, Joffe R, Goldstein V, Sarov I
Med Microbiol Immunol. 1978 Nov 17;166(1-4):177-86. doi: 10.1007/BF02121148.
An enzyme assay for the detection of antibodies to varicella-zoster membrane antigen (IPAMA) and a solid phase radioimmunoassay (RIA) which utilizes infected cell lysates as antigen are described. The results have been compared with those obtained by the indirect fluorescent antibody to membrane antigen (IFAMA) technique. It was found that IPAMA was equal in sensitivity to IFAMA. No cross-reactivity was found with other herpes group viruses. The IPAMA appears to give objective results, is easily and rapidly performed, and is recommended as a routine test for serological diagnosis of varicella-zoster infection. The RIA method is about 100 times more sensitive than IPAMA and IFAMA. The RIA is specific and has the potential of determining lower levels of antibody than other serological methods currently in use.
本文描述了一种用于检测水痘-带状疱疹膜抗原抗体的酶试验（IPAMA）以及一种利用感染细胞裂解物作为抗原的固相放射免疫测定法（RIA）。已将结果与通过间接荧光膜抗原抗体技术（IFAMA）获得的结果进行了比较。发现IPAMA的敏感性与IFAMA相当。未发现与其他疱疹病毒组病毒有交叉反应。IPAMA似乎能给出客观结果，操作简便快捷，推荐作为水痘-带状疱疹感染血清学诊断的常规检测方法。RIA方法的敏感性比IPAMA和IFAMA高约100倍。RIA具有特异性，并且有可能检测到比目前使用的其他血清学方法更低水平的抗体。
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