Wlodek M E, Harding R, Thorburn G D
Department of Physiology, Monash University, Clayton, Victoria, Australia.
Am J Obstet Gynecol. 1994 Jan;170(1 Pt 1):186-95. doi: 10.1016/s0002-9378(94)70406-6.
Our aim was to determine the effects of blocking prostaglandin synthesis, by infusion of indomethacin into the fetal circulation, on factors regulating amniotic fluid volume and on plasma composition in the mother and fetus.
Indomethacin was administered to fetal sheep during 8 hours at 124.0 +/- 1.2 days of gestation (n = 7) and at 134.7 +/- 0.8 days of gestation (n = 7) (term approximately 147 days). Vehicle infusions were performed at 128.8 +/- 1.4 days of gestation (n = 5).
Fetal urine production was significantly reduced and both fetal urine osmolality and plasma arginine vasopressin concentrations were significantly elevated in response to indomethacin infusions at both gestational ages. Fetal blood and urine lactate concentrations were elevated in response to indomethacin. Later in gestation, fetal lung liquid flow was significantly changed from a net efflux to a net influx. There was no significant effect of indomethacin on the volume of fluid swallowed by the fetus.
Inhibition of fetal prostaglandin synthesis profoundly reduces the production of the two major fetal fluids contributing to amniotic fluid, namely, urine and lung liquid. These findings may explain why indomethacin reduces amniotic fluid volume.
我们的目的是通过向胎儿循环中输注吲哚美辛来确定阻断前列腺素合成对调节羊水量的因素以及对母体和胎儿血浆成分的影响。
在妊娠124.0±1.2天（n = 7）和妊娠134.7±0.8天（n = 7）（足月约147天）时，对胎羊进行8小时的吲哚美辛给药。在妊娠128.8±1.4天（n = 5）时进行载体输注。
在两个胎龄时，对吲哚美辛输注的反应是胎儿尿量显著减少，胎儿尿渗透压和血浆精氨酸加压素浓度均显著升高。吲哚美辛使胎儿血液和尿液中的乳酸浓度升高。在妊娠后期，胎儿肺液流动从净流出显著转变为净流入。吲哚美辛对胎儿吞咽的液体量没有显著影响。
抑制胎儿前列腺素合成可显著减少对羊水量有贡献的两种主要胎儿液体，即尿液和肺液的产生。这些发现可能解释了为什么吲哚美辛会减少羊水量。
