Lauterborn J C, Tran T M, Isackson P J, Gall C M
Department of Anatomy and Neurobiology, University of California, Irvine 92715.
Neuroreport. 1993 Dec 13;5(3):273-6. doi: 10.1097/00001756-199312000-00023.
Isotopic and colorimetric in situ hybridization techniques were combined to determine if nerve growth factor (NGF) mRNA is colocalized with mRNA for the GABA biosynthetic enzyme glutamic acid decarboxylase (GAD) in adult rat hippocampus. Quantification of neurons labeled with both 35S-labeled GAD67 mRNA and digoxigenin-labeled NGF cRNA determined that of the NGF cRNA-labeled neurons, 97% within regions CA3-CA1, and 88% within the hilus, were also labeled with GAD67 cRNA. Overall, 47% of the total population of GAD67 cRNA labeled cells were NGF cRNA positive. The greater portion of stratum granulosum was lightly labeled by the NGF cRNA alone. The results indicate that, excepting stratum granulosum, NGF is predominantly synthesized by GABAergic neurons in rat hippocampus.
结合同位素和比色原位杂交技术，以确定在成年大鼠海马中神经生长因子（NGF）mRNA是否与γ-氨基丁酸（GABA）生物合成酶谷氨酸脱羧酶（GAD）的mRNA共定位。对用35S标记的GAD67 mRNA和地高辛标记的NGF cRNA标记的神经元进行定量分析，结果显示，在CA3-CA1区域内，97%的NGF cRNA标记神经元也被GAD67 cRNA标记；在海马门区内，这一比例为88%。总体而言，GAD67 cRNA标记细胞总数的47%为NGF cRNA阳性。颗粒层的大部分区域仅被NGF cRNA轻度标记。结果表明，除颗粒层外，大鼠海马中的NGF主要由GABA能神经元合成。
