制霉菌素穿孔膜片钳记录揭示大鼠皮层神经元中KA介导的外向电流。

Nystatin-perforated patch recordings disclose KA-operated outward currents in rat cortical neurons.

作者信息

Omura T, Munakata M, Akaike N

机构信息

Department of Neurophysiology, School of Medicine, Tohoku University, Sendai, Japan.

出版信息

Brain Res. 1993 Nov 12;627(2):345-8. doi: 10.1016/0006-8993(93)90340-s.

DOI:10.1016/0006-8993(93)90340-s

PMID:8298978

Abstract

Kainate (KA)-induced responses were studied in acutely dissociated rat cortical neurons. KA elicited the inward currents with conventional whole-cell and nystatin-perforated patch recordings under voltage-clamp condition. An additional outward current was observed only with the perforated patch recording. The outward current was due to the activation of K+ current by Ca2+ passing through the KA channels. This K+ channel was sensitive to both iberiotoxin and tetraethylammonium (TEA)-Cl. This K+ channel may serve to limit the depolarization during excessive KA-mediated excitation.

摘要

在急性分离的大鼠皮层神经元中研究了海人酸（KA）诱导的反应。在电压钳条件下，使用传统的全细胞膜片钳和制霉菌素穿孔膜片钳记录，KA可引发内向电流。仅在穿孔膜片钳记录中观察到额外的外向电流。该外向电流是由于Ca2+通过KA通道激活了K+电流所致。这种K+通道对iberiotoxin和四乙铵（TEA）-Cl均敏感。这种K+通道可能有助于限制KA介导的过度兴奋期间的去极化。