Niimi N, Wajjwalku W, Ando Y, Tomida S, Takeuchi M, Ueda M, Kaneda T, Yoshikai Y
Department of Oral Surgery, Nagoya University School of Medicine, Japan.
Eur J Immunol. 1994 Feb;24(2):488-91. doi: 10.1002/eji.1830240235.
A gene-encoding ligand for deletion of T cells bearing TcRV beta 6 and V beta 8.1 cosegregates a new mammary tumor provirus locus, Mtv-50 in NC mice. The sequence of the open reading frame (ORF) in the 3' long terminal repeat (LTR) of Mtv-50 was strikingly similar to those of Mtv-7, Mtv-43 and exogenous mouse mammary tumor virus (SW) with properties of minor lymphocyte stimulating antigen 1a. Consistent with previous reports, clonal deletion of mature thymocytes bearing TcRV beta 6 was defective during the early postnatal period of mice carrying Mtv-50. Appreciable levels of mRNA corresponding to common Mtv ORF and Mtv-6 ORF were expressed in the neonatal thymus, while little, if any, mRNA corresponding to Mtv-50 ORF was detected in the thymus at the early postnatal stage. Delay in expression of Mtv-50 ORF during the postnatal period may be responsible for the failure of clonal deletion of V beta 6-T cells in the early postnatal life of mice carrying Mtv-50.
一种编码配体的基因，可用于删除携带TcRVβ6和Vβ8.1的T细胞，该基因与NC小鼠中的一个新的乳腺肿瘤前病毒位点Mtv-50共分离。Mtv-50的3'长末端重复序列（LTR）中的开放阅读框（ORF）序列与Mtv-7、Mtv-43和具有次要淋巴细胞刺激抗原1a特性的外源性小鼠乳腺肿瘤病毒（SW）的序列惊人地相似。与先前的报道一致，携带Mtv-50的小鼠在出生后早期，携带TcRVβ6的成熟胸腺细胞的克隆性删除存在缺陷。在新生胸腺中表达了与常见Mtv ORF和Mtv-6 ORF相对应的可观水平的mRNA，而在出生后早期阶段的胸腺中，几乎未检测到与Mtv-50 ORF相对应的mRNA（如果有的话）。出生后时期Mtv-50 ORF表达的延迟可能是携带Mtv-50的小鼠出生后早期Vβ6-T细胞克隆性删除失败的原因。
